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p47 rogfp pal  (Addgene inc)


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    Addgene inc p47 rogfp pal
    P47 Rogfp Pal, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p47+rogfp+pal/GFP%2ECre+empty+vector+(Plasmid+%2320781)/pm37980559-270-178-176
    Average 91 stars, based on 27 article reviews
    p47 rogfp pal - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    Titanium Dioxide:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    Gene Expression:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    RNA Sequencing:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    Phospho-proteomics:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    Recombinant:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    shRNA:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    Control:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023

    Software:

    Article Title: Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dihydroethidium Merck Cat# D7008 Critical commercial assays Zombie Violet Fixable Viability Kit BioLegend Cat #423114 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat #34580 Permeabilization buffer Thermo Fisher Scientific Cat# 00-8333-56 RNeasy Plus Micro Kit Qiagen Cat# 74034 Agilent TapeStation High Sensitivity tape Agilent Cat# 5067- 5584 SuperScript III First-Strand Synthesis SuperMix Thermo Fisher Scientific Cat# 18080400 iQTM SYBR Green Supermix Bio-Rad Cat# 1708880 Titanium dioxide beads Thermo Fisher Scientific Cat# A32993 Ion AmpliSeqTM Transcriptome Mouse Gene Expression Kit Thermo Fisher Scientific Cat# A36553 Deposited data RNA-seq This paper https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE244773 Phosphoproteomics This paper https://ftp.pride.ebi.ac.uk/pride/data/archive/ 2023/10/PXD045719/ Experimental models: Cell lines HMC3 ATCC Cat# CRL-3304, RRID:CVCL_II76 Experimental models: Organisms/strains Mouse: Rac1fl/fl:Cx3cr1CreER+ This paper N/A Mouse: B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2) Litt/WganJ (herein Cx3cr1CreER EYFP) The Jackson Laboratory Cat# JAX:021160; RRID:IMSR_JAX:021160 Mouse: Rac1tm1Brak (herein Rac1fl/fl) MGI RRID:MGI:3665264 Oligonucleotides Primers in Table S11 This paper N/A Recombinant DNA Daglas-pm1 Sato et al.39 N/A pcDNA-D1ER Palmer et al.40 Addgene #36325 CMV-R-GECO1 Wu et al.41 Addgene #32444 CKAR Violin et al.42 Addgene #14860 GFP.Cre Gift from Tyler Jacks Addgene #20781 p47-roGFP Pal et al.32 N/A pLKO-empty Karabiyik et al.66 N/A pLKO-Rac1 shRNA Karabiyik et al.66 N/A scrambled-mCherry shRNA control Karabiyik et al.66 N/A mCherry-Rac1 shRNA Karabiyik et al.66 N/A pRK5-myc-Rac1-wt Gift from Gary Bokoch Addgene #12985 pRK5-myc-Rac1-T17N Gift from Gary Bokoch Addgene #12984 pGP-CMV-GCaMP6f Chen et al.45 Addgene #40755 mCherry-Lifeact-7 Gift from Michael Davidson Addgene #54491 Software and algorithms Fiji Schindelin et al.67 RRID:SCR_002285 Transcriptome Analysis Console Thermo Fisher Scientific RRID:SCR_016519 Proteome Discoverer Thermo Fisher Scientific RRID:SCR_014477 GraphPad Prism GraphPad Software RRID:SCR_002798 Adobe Illustrator Adobe RRID:SCR_010279 Imaris Oxford Instruments RRID:SCR_007370 (Continued on next page) 20 Cell Reports 42, 113447, December 26, 2023



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    p47 rogfp pal  (Addgene inc)
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    P47 Rogfp Pal, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p47+rogfp+pal/GFP%2ECre+empty+vector+(Plasmid+%2320781)/pm37980559-270-178-176
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